Our ssDNA services.

Modified ssDNA partnership programme.

Internal chemistry at kilobase length

Partner with Moligo to develop long ssDNA with modified nucleotides distributed throughout the strand. Our modification technology is available to selected partners through a co-development programme with an agreed research scope, not an off-the-shelf modification service.

Together, we assess chemistry feasibility, select the base channel and substitution fraction, and agree characterisation and application testing for the construct. Exact positions and regional modification patterns are not controlled by this process.

A matched performance advantage over unmodified long ssDNA has not yet been demonstrated.

Linear ssDNA

Your sequence.
At gene length.

Custom HDR donor templates

Put your donor design into a single strand, with custom sequences up to 10 kb and no added scar sequences.

For research teams developing HDR donors and other sequence-defined tools. In vitro enzymatic synthesis supports long constructs, including challenging GC-rich regions, repeats and secondary structures, subject to construct review.

Product properties

Format

Linear single-stranded DNA

Length

Custom sequences up to 10 kb

Sequence

Sequence-verified; no scar sequences

Quality checks

Gel, Sanger alignment, S1 nuclease and absorbance testing

Reorders

Lifetime template storage at no charge

To order, send your sequence, target length, quantity and intended application. Confirm the construct specification and delivery requirements with our team.

Circular ssDNA

A continuous strand.
No free ends.

Circular donors for non-viral gene insertion research

Combine a custom donor sequence with a closed circular format. Explore topology as a design choice for your gene-insertion workflow.

Moligo’s collaboration with Cellectis evaluated circular versus linear donors in HSPCs. The study supports the circular format under its tested conditions; it does not establish an advantage for chemically modified circular DNA.

Product properties

Format

Circular single-stranded DNA

Length

Custom sequences up to 10 kb

Sequence

Sequence-verified; no scar sequences

Format verification

S1 nuclease digestion and exonuclease-resistance testing

Reorders

Lifetime template storage at no charge

To order, specify circular format and share your sequence, quantity and application. Review the QC criteria and agree the specification for your construct.

Off-the-shelf sequences.

Start with an existing AAV-like or gene-editing construct. Sequences are available on request.